Identifying Organic Compounds Lab free essay sample

The purpose of washing the test tubes thoroughly is so you don’t mix substances, which can cause your results to be inaccurate. Hypotheses: 1. If the test samples contain Protein, then in the presence of Biuret the color will turn to yellow, green, or lavender. 2. If the test samples contain Carbohydrates, then in the presence of Benedict’s color will turn to â€Å"traffic light colors† which consist or orange, red, yellow, and sometimes green. 3. If the test samples test positive for starches, then in the presence of Iodine, their colors if the test sample contains sugars, then the iodine will change from yellow-brown to blue-black. . If the test samples show the presence of lipids, the when the substance is placed onto a brown paper bag and sits for 10 minutes, then a translucent or opaque spot will appear. Materials: * 8 test tubes * test tube rack * test tube holder * glass stirring rod * sharpie marker * how water bath * iodine solution * brown paper bag * B iuret reagent * benedicts solution * 20 ml each: 1. We will write a custom essay sample on Identifying Organic Compounds Lab or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page honey solution 2. egg white and water solution 3. gelatin and water solution 4. melted butter and water solution 5. apple juice and water solution 6. distilled water 7. unknown solution Procedures: Obtain materials from the basket at the back of the class. Use the Sharpie maker to label the test tubes 1-8. Note the corresponding solutions to be placed in the tubes from the material section of the lab. 2. Fill each test tube with 5 ml of the substance indicated on the test tube. (Approximately one â€Å"pinkie† joint) 3. Divide a piece of brown paper bag into 8 individual sections #1Honey| #2 Egg white| #3Gelatin| #4 Butter| #5Apple juice| #6Corn Oil| #7Water| #8Unknown| 4. In each section, using the glass-stirring rod, rub a small amount of food on to the paper bag in the appropriate section until a â€Å"wet† spot appears. The spot on the bottom would be corn oil #6 because it did contain lipids. 2. If water tested positive for organic compounds you can conclude that the water is not pure distilled water, or that the test tube may have been not washed properly and was mixed with another substance, 3. My experiment supported my hypothesis because it showed that the lipids did make the paper translucent, the starch did turn from a yellow-brown to a very dark blue-black, the sugars did turn â€Å"Traffic light colors† which are orange, red, yellow, and sometimes green and also the proteins did turn to â€Å"Easter egg colors† which where lavender, yellow, or green.